FIG. 3.
Activation of CMKA and CMKB by A. nidulans and heterologous CaMs. The specific activity of hexa-histidine-tagged bacterially expressed and purified CMKA (A) and CMKB (B) was measured at different concentrations of purified AnCaM (closed square), VCaM (closed circle), ScCaM (closed triangle), or 1234 (closed diamond) CaM as described in Materials and Methods. The graphs are representative of three independent experiments.