Complementation of A. nidulans by heterologous CaMs. AlcCaM strains expressing the ectopically expressed AnCaM (open squares), VCaM (closed circles), ScCaM (open circles), and 1234 (closed triangles) were inoculated onto either MMG (A) or MMD (B) agar plates, and their colony diameters were measured over a period of 5 days. A nutritionally complemented pAL5#1 strain (open triangles) was used to represent wild-type A. nidulans (positive control), and the nutritionally complemented AlcCaM strain (closed squares) was used as a negative control.