Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HEK293 human kidney(embryonic) |
ECACC (Sigma) | #85120602 RRID:CVCL_0045 |
Batch No: 18E026 |
| Cell line (Homo sapiens) | HT29 Caucasian colon adenocarcinoma grade II |
ECACC (Sigma) | #91072201 RRID:CVCL_0320 |
Batch No: 09K003 |
| Cell line (Homo sapiens) | HepG2 human hepatocyte carcinoma |
ECACC (Sigma) | #85011430 RRID:CVCL_0027 |
Batch No: 19B009 |
| Cell line (Mus musculus) | HL1 mouse cardiomyocyte cell line, atrial |
Merck | # SCC065 RRID:CVCL_0303 |
Batch No: RD1601001 |
| Cell line (Homo sapiens) | HEK293TPalmGFP human kidney(embryonic) expressing palmitoylated GFP |
Kind gift of Charles Lai https://doi.org:10.103 8/ncomms8029 |
Resorted before MCB preparation | |
| Cell line (Homo sapiens) | HEK293T- PalmGFPLC3RFP human kidney(embryonic) expressing palmitoylated GFP and RFP tagged LC3 |
This paper | See Materials and methods | |
| Cell line (Mus musculus) | BMMC bone marrow-derived mast cells | Primary cell culture https://doi.org/10.1 002/jev2.12023 |
||
| Cell line (Rattus norvegicus) | H9C2 (2-1) rat cardiovascular , Myoblast | ECACC (Sigma) | #88092904, RRID:CVCL_0286 | Batch No: 17A028 |
| Transfected construct | LentiBrite RFP-LC3 Lentiviral Biosensor |
Merck | 17-10143 | Batch No: 3530171 |
| Biological sample (Mus musculus) | own animal house | C57BL/6 RRID:MGI:2159769 |
male, 12 weeks of age | |
| Antibody | rabbit polyclonal anti-CD63 (Cterminal) | Sigma/Merck | SAB2109138 | IF (1:200) WB (1:500) |
| Antibody | mouse monoclonal anti-CD63 | Santa Cruz Biotechnology |
MX-49.129.5 clone: sc-5275 RRID:AB_627877 |
IF (1:200) TEM (1:50) WB (1:1000) |
| Antibody | rabbit polyclonal anti-CD81 | Sigma/Merck | SAB3500454 RRID:AB_10640751 |
IF (1:200) WB (1:2500) |
| Antibody | mouse monoclonal anti-CD81 | Invitrogen | MA5-13548 clone: 1.3.3.22 RRID:AB_10987151 |
IF (1:100) WB (1:100) |
| Antibody | rabbit polyclonal anti-TSG101 | Sigma/Merck | HPA006161 RRID:AB_1080408 |
IF (1:200) WB (1:1000) |
| Antibody | rabbit polyclonal anti-ALIX (Cterminal) | Sigma/Merck | SAB420047 | IF (1:200) WB (1:1000) |
| Antibody | rabbit monoclonal anti-LC3B | Sigma/Merck | ZRB100 clone: 12K5 |
IF (1:200) TEM (1:50) WB (1:1000) |
| Antibody | rabbit monoclonal anti-LC3A | Sigma/Merck | ZRB1125 clone: 3J12 |
IF (1:200) WB (1:1000) |
| Antibody | rabbit polyclonal anti-TSPAN4 | Bioss | BS-9413R | IF (1:200) |
| Antibody | mouse monoclonal anti-Rab7 | Sigma/Merck | R8779 clone: Rab7117 RRID:AB_609910 |
IF (1:200) WB (1:1000) |
| Antibody | mouse monoclonal anti-α-tubulin | Sigma/Merck | T9026 clone: DM1A RRID:AB_477593 |
IF (1:200) |
| Antibody | mouse monoclonal anti-GFP | Sigma/Merck | G6539 clone: GFP-20 RRID:AB_259941 |
IF (1:200) WB (1:1000) |
| Antibody | mouse monoclonal anti-RFP | Invitrogen | MA5-15257 clone: RF5R RRID:AB_10999796 |
WB (1:1000) |
| Antibody | goat antimouse IgGATTO550 | Sigma/Merck | 43394 RRID:AB_1137651 |
IF (1:1000) |
| Antibody | goat antirabbit IgG- ATTO647N |
Sigma/Merck | 40839 RRID:AB_1137669 |
IF (1:1000) |
| Antibody | goat antimouse Star 635P | Abberior | ST635P-1001–500UG RRID:AB_2893232 |
IF (1:500) |
| Antibody | goat antirabbit Star 580 | Abberior | ST580-1002-500UG RRID:AB_2910107 |
IF (1:500) |
| Antibody | goat polyclonal anti-rabbit IgG Fc (HRP) |
abcam | ab97200 RRID:AB_10679899 |
WB (1:10,000) |
| Antibody | goat polyclonal anti-mouse IgG Fc (HRP) | abcam | ab97265 RRID:AB_10680426 |
WB (1:10,000) |
| Antibody | goat polyclonal anti-rabbit IgG (whole molecule) 10 nm gold preadsorbed | abcam | ab27234 RRID:AB_954427 |
TEM (1:50) |
| Antibody | goat polyclonal anti-mouse IgG (whole molecule) 5 nm gold preadsorbed | Sigma/Merck | G7527 RRID:AB_259955 |
TEM (1:50) |
| Other | CF488A conjugated Wheat Germ Agglutinin (WGA) | Biotium | 29022-1 | Lot Number: 21C0224-1149057 |
| Chemical compound, drug | Bafilomycin A1 | Sigma/Merck | B1793 | Lot Number: 0000190389 |
| Chemical compound, drug | Colchicine | Serva | 77120.02 | Lot Number: 190300 |
| Chemical compound, drug | Chloroquine diphosphate | Invitrogen | P36236 C | Lot Number: 2441325 |
| Chemical compound, drug | Rapamycin | Sigma/Merck | R0395 | Lot Number: 0000084976 |
| Chemical compound, drug | Cytochalasin B | Sigma | C2743 | Lot Number: 037M4083V |
| Chemical compound, drug | FBS | Biosera | FB-1090/500 | Lot Number: 015BS575 |
| Other | TFF Easy | HansaBioMed Life Sciences | HBM-TFF/1 | |
| Software, algorithm | LASX | Leica | Leica Application Suite X 3.5.5.19976 |
|
| Software, algorithm | ZEN Blue | Zeiss | ZEN 2.3 lite | |
| Software, algorithm | iTEM | Olympus | iTEM 5.1 | |
| Software, algorithm | ImageJ | https://imagej.n et/ij/ | v1.54g | |
| Software, algorithm | Prism9 | GraphPad | GraphPad Prism 9.4.1 | |
| Software, algorithm | BioRender | https://www.biorender.com/ |