FIG. 3.

The srb11Δ meiotic defect is independent of checkpoint pathways. Wild-type strain RSY335 (SRB11), mutant strain RSY389 (srb11Δ), or double-mutant strain RSY504 (srb11Δ rad17Δ) was induced to enter meiosis, and samples were taken at the indicated times. The samples were fixed, stained with DAPI, and examined for the production of binucleated and tetranucleated cells. A total of 200 cells were counted for each time point.(Top) Total percentage of the population completing either meiotic division plotted against time in sporulation medium. (Bottom) Percentage of the population containing only binucleated cells.