TABLE 3.

IME2 overexpression partially suppresses the srb11 meiotic defect^a

Overexpression plasmid	% of cells that were
	Mononucleated	Binucleated	Tri- and tetranucleated	Totally sporulated
SRB11	17	4	79	83
Vector	21	42	37	79
IME2	17 ± 6	21 ± 1	62 ± 5	83 ± 4

^aRSY389 (srb11Δ) was transformed with the indicated plasmids: SRB11, pKC342 (10); vector, pRS425; pMR1, IME2 inserted into a 2μm high-copy-number vector (see Materials and Methods for details). Transformants were subjected to a standard sporulation regimen in liquid medium. Cells were fixed and stained with DAPI 24 h after a shift to sporulation medium. Three independent cultures harboring pMR1 were assayed; data are means and standard deviations.