Skip to main content
. 2005 Jul 25;33(13):4128–4139. doi: 10.1093/nar/gki727

Figure 3.

Figure 3

Western blot analysis of endogeneous Translin in S.pombe cell extracts. The western blot procedure was carried out, as described in Materials and Methods. 150 μg of total protein from wild-type or mutant S.pombe cells were loaded per lane. Recombinant S.pombe Translin served as a positive control. Polyclonal anti-S.pombe Translin antibodies were used to probe the membrane. Lane 1, wild-type strain (FY370); lane 2, tsn+ derivative of a heterozygous diploid tsn+/tsn−; lane 3, tsn− derivative of the heterozygous diploid tsn+/tsn−; lane 4, trax− mutant; lane 5, tsn−trax− mutant; lane 6, Recombinant S.pombe Translin. Closed arrowhead marks the position of endogenous Translin. Open arrowhead marks the position of recombinant Translin. Equal sample loading was confirmed by Coomassie blue staining. The mutant genotypes are described in the legend to Figure 4 and in the text.