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. 2005 Jul 25;33(13):4128–4139. doi: 10.1093/nar/gki727

Figure 7.

Figure 7

Gel shift competition assays designed to determine the relative affinities of the human and the S.pombe Translins for the oligoribonucleotide (GU)15 and the oligodeoxynucleotide d(GT)15. Recombinant S.pombe Translin or human Translin was incubated with 1 nM of a 32P-d(GT)15 probe, or a 32P-(GU)15 probe, in the presence of increasing concentrations of the indicated unlabeled competitors. Gel mobility shift assays were performed, as described in Materials and Methods. Only the upper parts of the gels, which contain the retarded band, are shown. (A) S.pombe Translin, 32P-d(GT)15 probe. (B) Human Translin, 32P-d(GT)15 probe. (C) S.pombe Translin, 32P-(GU)15 probe. (D) Human Translin, 32P-(GU)15 probe. The numbers at the bottom of each part represent the percentage of bound probe as a function of the concentration of competitor, relative to the value obtained in the absence of competitor, which was designated as 100%.