Table 1.
S.pombe strains used in the present study
| Strain | Genotype | Source |
|---|---|---|
| FY370 | h+, ade6-DN/N, ura4-D18 | (44) |
| AP137 | mat1-PΔ17::leu2, leu1-32, ura4-D18, ade6-210, his2 | (45) |
| SP1124 | mat1-Msmt0, L(XbaI) ::ura4+, ade-216, ura4-D18 | (45) |
| SP1124fa | mat1-Msmt0, L(XbaI) ::ura4−, ade-216, ura4-D18 | This study |
| ΔTRAX | h−, Δtraxb::kanR, leu1-32, ura4-D18, ade6-216 | (23) |
| Spmd49c | mat1-PΔ17::leu2, mat1-Msmt0, leu1-32, ura4-D18, L(XbaI)::ura4−, Δtsn::ura4+, ade6-210, ade-216, his2 | This study |
| Spm14d | mat1-PΔ17::leu2, Δtsn::ura4+, leu1-32, ura4-D18, ade6-210 | This study |
| Spm28a | h−, Δtsn::ura4, Δtrax::kanR, leu1-32, ura4-D18, ade6-210 | This study |
aSelected from SP1124 by growth in medium containing 5FOA.
btrax designates the gene encoding the TRAX protein. In (23) the TRAX open reading frame is designated spcc736.09c.
cTranslin heterozygous diploid.