Results of 989-bp β-globin fragment PCR amplification of DNA extracted from aliquots of buccal cells. The arrow indicates the 989-bp fragment. Lanes 3–12 and 14 (3,4,8, and 10–12 are E-beam irradiated, and 5–7, 9, and 14 are untreated) are test specimens, lanes 13 and 15 are blank controls, lane 2 is a positive amplification control (K562 cell DNA), and lanes 1 and 16 are molecular size standards. Lanes 5, 6, 9, and 14 were rated as “positive,” lanes 3, 4, 7, 8, 10, and 11 were rated as “weak positive,” and lanes 12, 13, and 15 were rated as “negative.”