Table 1.
Results for Paired Aliquots(n=29) |
|||
Method | Untreated | Irradiated | Pa |
DNA yields, medianb: | |||
Spectrophotometric (260 nm) | 12.3 μg | 5.9 μg | .002 |
PicoGreen | 8.9 μg | 3.9 μg | .01 |
TaqMan | 7.6 μg | 3.7 μg | <.0005 |
Genomic integrity (maximum size)c: | |||
⩾23,130 bp | 93.1% | .0% | |
<23,130 bp | 6.9% | 31.0% | |
<6,557 bp | .0% | 65.5% | |
<564 bp | .0% | 3.5% | <.0005 |
PCR Amplification of a 989-bp β-globin: | |||
Positive | 93% | 0% | |
Weak positive | 7% | 97% | |
Negative | 0% | 3% | <.0005 |
PCR Amplification of a 536-bp β-globin: | |||
Positive | 100% | 100% | |
Weak positive | 0% | 0% | |
Negative | 0% | 0% | 1.0 |
PCR Amplification of a 268-bp β-globin: | |||
Positive | 100% | 100% | |
Weak positive | 0% | 0% | |
Negative | 0% | 0% | 1.0 |
Wilcoxon matched-pair sign-rank test (continuous) and Pearson χ2 test (categorical).
Yield per aliquot. Each aliquot represents one-half of the whole mouthwash specimen.
Electrophoretic migration of specimen DNA was compared with known molecular size markers (Ready-Load λ DNA/HindIII Fragments [Invitrogen]) and rated for the maximum fragment size as “no visible DNA,” “<564 bp,” “<6,557 bp,” “<23,130 bp,” or “⩾23,130 bp.”