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. 2003 Aug 13;73(3):646–651. doi: 10.1086/378077

Table 1.

Summary of DNA Yields, DNA Integrity Measurement, and Outcomes of PCR Amplification of β-Globin Fragments of Lengths 989 bp, 536 bp, and 268 bp

Results for Paired Aliquots(n=29)
Method Untreated Irradiated Pa
DNA yields, medianb:
 Spectrophotometric (260 nm) 12.3 μg 5.9 μg .002
 PicoGreen 8.9 μg 3.9 μg .01
TaqMan 7.6 μg 3.7 μg <.0005
Genomic integrity (maximum size)c:
 ⩾23,130 bp 93.1% .0%
 <23,130 bp 6.9% 31.0%
 <6,557 bp .0% 65.5%
 <564 bp .0% 3.5% <.0005
PCR Amplification of a 989-bp β-globin:
 Positive 93% 0%
 Weak positive 7% 97%
 Negative 0% 3% <.0005
PCR Amplification of a 536-bp β-globin:
 Positive 100% 100%
 Weak positive 0% 0%
 Negative 0% 0% 1.0
PCR Amplification of a 268-bp β-globin:
 Positive 100% 100%
 Weak positive 0% 0%
 Negative 0% 0% 1.0
a

Wilcoxon matched-pair sign-rank test (continuous) and Pearson χ2 test (categorical).

b

Yield per aliquot. Each aliquot represents one-half of the whole mouthwash specimen.

c

Electrophoretic migration of specimen DNA was compared with known molecular size markers (Ready-Load λ DNA/HindIII Fragments [Invitrogen]) and rated for the maximum fragment size as “no visible DNA,” “<564 bp,” “<6,557 bp,” “<23,130 bp,” or “⩾23,130 bp.”