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. 2005 Jul 26;389(Pt 3):629–636. doi: 10.1042/BJ20050067

Figure 5. Phosphorylation and ubiquitination of EGFR and PDGFRs in the presence of Src-kinase inhibitors.

Figure 5

HER14 cells were serum-starved for 16 h, preincubated either with 2 μM SU6656 or vehicle for 1 h or with 2.5 μM PP1 for 15 min and stimulated with (a, c) 100 ng/ml EGF or (b, d) 30 ng/ml PDGF, for 8 min at 37 °C. Cell lysates were subjected to immunoprecipitation with (a, c) anti-EGFR or (b, d) anti-PDGFR antibody. Phosphorylation and ubiquitination were assessed with PY20 antibody (P-Tyr) and anti-ubiquitin antibody respectively. The blots were reprobed with anti-EGFR or anti-PDGFR antibody (bottom panels).