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. 2005 Jul 26;389(Pt 3):629–636. doi: 10.1042/BJ20050067

Figure 6. Co-localization of internalized EGFR with Hrs in the presence of Src-kinase inhibitors.

Figure 6

HeLa cells were seeded on to coverslips, serum-starved for 16 h, preincubated either with 2 μM SU6656 or vehicle for 1 h or with 2.5 μM PP1 for 15 min, and stimulated with 100 ng/ml EGF for 8 min at 37 °C. The cells were fixed with methanol and stained with anti-EGFR (EGFR, shown in green) and anti-Hrs antibody (Hrs, shown in red) followed by secondary antibodies coupled with Alexa Fluor 488 and Alexa Fluor 594 respectively. All panels show a single confocal section. Scale bars, 20 μm.