Figure 4. Sensorgram of a typical SPR experiment.

A Biacore L1 chip was washed several times with detergent (step 1) before POPC/POPS (80/20 mol%) vesicles (step 2) were injected. After adsorption and fusion of vesicles a short pulse of NaOH (10 mM) was applied (step 3) to remove multilayers or loosely bound vesicles. For the blocking of non-specific binding sites, BSA (1 mg/ml) was added (step 4). The chips were subsequently used to study the interaction with β₂GPI. Open bars indicate washing with running buffer.