Table 1. Kinetic constants of the interaction of β2GPI with POPC/POPS lipid bilayers at different phosphatidylserine contents and the effect of Ca2+ (two-state reaction model).
Phosphatidylserine content (mol%) | [Ca2+] (mM) | kon, 1 (M−1·s−1×104) | koff, 1 (s−1×10−1) | kon, 2 (s−1×10−2) | koff, 2 (s−1×10−3) | KD (nM) |
---|---|---|---|---|---|---|
10 | 3 | 1.67±0.23 | 3.35±1.13 | 3.93±2.28 | 1.80±0.04 | 1078±224 |
20 | 3 | 4.62±0.77 | 1.64±0.08 | 2.04±0.19 | 2.05±0.25 | 339±22 |
20 | 0 | 10.53±0.04 | 1.99±0.19 | 0.97±0.02 | 0.94±0.44 | 139±30 |