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. 2005 Jul 26;389(Pt 3):705–716. doi: 10.1042/BJ20050214

Figure 5. Cartilage-specific occupancy of the Pe1 and SII elements in genomic footprinting.

Figure 5

(A) Footprints are shown between positions –169 and –125 on the lower strand of the DNA. AG and CT are Maxam–Gilbert control sequences. DNA from CEC and CEF cultures treated in vivo (v) with DMS (open and closed boxes) or UV light (open and closed circles) is compared with the in vitro (t) DNA samples treated with these reagents after isolation from CEC and CEF. Differences in the modification patterns between ‘v’ and ‘t’ treatments, visible as hyper-reactivities (large and small closed circles or closed boxes) or protections (large and small open circles or open boxes), indicate in vivo DNA–protein contacts at specific sequences. (B) Summary of the in vivo footprinting data is shown on both strands. The previously identified NFI-binding site in the SII element and the inverted repeat harbouring the putative paired Sox-binding sites are boxed.