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. 2005 Jul 26;389(Pt 3):797–802. doi: 10.1042/BJ20050405

Figure 3. L-Cysteine is unique in mobilizing the iron centre in IscA.

Figure 3

(A) The iron-loaded IscA (100 μM) was incubated with Tris (20 mM; pH 8.0), NaCl (200 mM), dithiothreitol (2 mM) and 1 mM each of 2-mercaptoethanol (BME), N-acetyl-L-cysteine (NAC), glutathione (GSH), L-cysteine (L-Cys) and D-cysteine (D-Cys) respectively at 37 °C for 20 min. The samples were then desalted using a HiTrap desalting column and subjected to UV–visible absorption measurements. (B) The EPR spectra of the same samples as prepared for (A) except that the proteins concentration was approx. 400 μM.