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. 2005 Jul 26;389(Pt 3):831–841. doi: 10.1042/BJ20050334

Figure 6. Effect of mutations in the Foxa sites A, B and C on the transactivation by Foxa2 of the rat glucagon gene promoter.

Figure 6

A schematic representation of wild-type (−350GluLuc) and mutated constructs shows the location of the Foxa site A (A), Foxa site B (B) and Foxa site C (C). The mutated site is marked by a cross. HepG2 cells were co-transfected with either −350GluLuc or mutated constructs (−350GluLuc-mtC, −350GluLuc-mtA, −350GluLuc-mtB, −350GluLuc-mtAB or −350GluLuc-mtABC) and the Foxa2 expression plasmid (450 ng/dish). The Foxa2-induced luciferase activity is expressed as a percentage of the mean value of the activity measured with −350GluLuc for each experiment. Values are means±S.E.M. for at least four independent experiments, each performed in duplicate.