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. 2005 Jul 26;389(Pt 3):853–861. doi: 10.1042/BJ20042019

Figure 4. Detection of GPI-anchored Arts in pcDNA3-cgArt1/2-transfected DT40 cells.

Figure 4

Stable transfection of the pcDNA3-cgArt1 or pcDNA3-cgArt2 construct into DT40 cells was performed. (A) Art assay and in situ zymographic analysis were performed with the lysates of pcDNA3-cgArt1/2 or pcDNA3 (empty vector)-transfected cells and purified lung Art. (B) Release by PI-PLC treatment of recombinant Arts from the membrane fractions of the transfected DT40 cells. The data are expressed as means±S.D. for four independent experiments.