Table 1. Properties of chicken Arts.
The activities of chicken Arts were measured by poly(L-arginine) assay. The PI-PLC-treated supernatants of the membrane fractions from DT40 cells transfected with the pcDNA3-cgArt1/2 constructs were used as the enzyme fractions. Molecular masses were determined by SDS/PAGE and in situ zymographic assay. For measurement of substrate specificity, incorporation of radioactivity into the substrates by purified Art6.1 was set as 100%; incorporation by cgArt1 or cgArt2 is shown as relative to this.
| cgArt1 | cgArt2 | Art6.1 | |
|---|---|---|---|
| Molecular mass (kDa) | 45 | 45 | 27.5 |
| IC50 for: | |||
| NaCl (mM) | 125 | 278 | 102 |
| β-NMN (μM) | 350 | 21 | >1000 |
| Substrate specificity (%) | |||
| Actin | 3 | 2 | 100 |
| p33 | 97 | 100 | 100 |
| Casein | 8 | 8 | 100 |
| Histone | 65 | 130 | 100 |