TABLE 5.

Nucleic acid detection in patients with histology and/or mycologic proven invasive aspergillosis

Gene	Methoda	Annealing temp (°C)	No. of cycles	Detection method(s)	Sampleb	No. with positive reaction/total no. of subjects (%)		Reference
						Patients with proven invasive aspergillosis	Controlsc
Alkaline protease	sPCR	63	42	EtBrd staining-Southern blotting	BAL fluid	4/4 (100.0)	6/46 (13.0)	202
26S ITS	sPCR	56	32	EtBr staining-Southern blotting	BAL fluid or other respiratory specimen	3/3 (100.0)	4/17 (23.5)	193
18S rRNA	sPCR	62	40	EtBr staining-Southern blotting	BAL fluid	4/4 (100.0)	0/14 (0)	138
5S rRNA	ISH				Tissue	12/12 (100.0)	0/18 (0)	145
Mitochodrial	cPCR	60	40	EtBr staining-hybridization	BAL fluid	3/3 (100.0)	12/49 (24.5)	17
18S rRNA	nPCR	50/65	30/30	EtBr staining-Southern blotting	Serum	14/20 (70.0)	0/20 (0)	243
Mitochondrial	cPCR	55	45	ELISA	BAL fluid	2/6 (33.3)	0/19 (0)	16
Mitochondrial	sPCR	58	40	ELISA	BAL fluid	3/3 (100.0)	0/57 (0)	95
18S rRNA	nPCR	50/65	30/30	EtBr staining	Serum	4/4 (100.0)		244
18S rRNA	sPCR	62	35	ELISA	Blood	7/7 (100.0)	0/10 (0)	126
18S rRNA	nPCR	57/60	40/30	EtBr staining	Serum	4/4 (100.0)		104
18S rRNA	nPCR	65/65	23/35	EtBr staining	Blood	6/6 (100.0)	1/188 (0.53)	186
18S rRNA	nPCR	65/65	23/35	EtBr staining	BAL fluid	6/6 (100.0)	4/188 (2.1)	186
Large rRNA	nPCR	58/58	31/31	EtBr staining	Serum	6/6 (100.0)	4/19 (21.1)	230

^aAbbreviations: sPCR, standard PCR; cPCR, competitive PCR; nPCR, nested PCR; ISH, in situ hybridization.

^bBAL, bronchoalveolar lavage.

^cIncluded healthy subjects and/or colonized patients and/or patients with other infections.

^dEtBr, ethidium bromide.