Figure 4. Far-UV CD spectra of AnxA13b as a function of pH.

Spectra were recorded at 20 °C using AnxA13b at 0.1 mg/ml in sodium citrate buffer solutions at decreasing pH values, as described in the Experimental section. Only representative spectra at pH 8.0, 4.1 and 2.3 are shown. The inset shows the plot of ellipticity values at 220 and 208 nm against pH (upper panel) and the ratio between these values (lower panel).