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. 2005 Jul 26;33(13):4191–4201. doi: 10.1093/nar/gki734

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Transcriptional repressive activity of GZF1. (A) Schematic representation of the reporter vectors. Five tandem repeats of GRE were cloned upstream of the luciferase gene in a pGL3-basic reporter vector (designated pGL3-5 × GRE). The reporter vector containing five GREs in the antisense direction was also constructed (pGL3-5 × rGRE). (B) Luciferase assay using the extracts from HEK293T cells transiently co-transfected with increasing amounts (50 and 100 ng) of an expression vector carrying full-length GZF1 cDNA (pSRα-GZF1) and pGL3, pGL3-5 × GRE or pGL3-5 × rGRE.