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. 2005 Jul 26;33(13):4191–4201. doi: 10.1093/nar/gki734

Figure 9.

Figure 9

(A) Schematic representation of a pGL3-basic reporter vector containing six copies of GRE(HOXA10) in both orientations upstream of the luciferase gene. We also synthesized 6×GRE(HOXA10-mut) in which the conserved TGCGC (sense orientation) or GCGCA (antisense orientation) motifs in 6×GRE(HOXA10) were changed to CATAT or ATATG, respectively. (B) Schematic representation of mutant GZF1 (Zn4m, Zn6m and Zn10m) in which two cysteines in zinc fingers 4, 6 or 10 were replaced with arginines. The wild-type and mutated fingers are shown as white and gray cylinders, respectively. The BTB/POZ domain is shown as a black cylinder. (C) Transcriptional repressive activity of GZF1 was markedly impaired by mutation of zinc finger 4 (Zn4m). Mutation of zinc finger 6 (Zn6m) moderately reduced the repressive activity of GZF1 but mutation of zinc finger 10 (Zn10m) showed no effect. Increased amounts of wild-type or mutant GZF1 (50, 100, 200 and 500 ng) were co-transfected with pGL3-6×GRE(HOXA10) or pGL3-6×GRE(HOXA10-mut).