TABLE 2.
Selected chronology of efforts to cultivate Cryptosporidium spp. in vitroa
| Cell line (ATCC no.) | Oocyst source(s) |
Cryptosporidium life cycle stage developmentb
|
Reference(s) | ||
|---|---|---|---|---|---|
| Asexual | Sexual | Oocysts | |||
| HRT | Bovine | Yes | ND | ND | 61 |
| Chicken CAM | Human, bovine | Yes | Yes | Yes | 16 |
| Human, passaged in a goat | Yes | NR | NR | 36 | |
| Chicken (C. baileyi) | Yes | Yes | Yes | 31 | |
| HFL | Human, passaged in a goat | Yes | Yes | Yes | 15 |
| PCK, PK-10 | Human, passaged in a goat | Yes | Yes | Yes | 15 |
| BHK (CRL-1632) | Human, passaged in a goat | Yes | NR | NR | 36 |
| MDCK (CCL-34) | Caprine | Yes | NR | NR | 32 |
| Bovine | Yes | Yes | Yes* | 25 | |
| Bovine | Yes | Yes | Yes | 47 | |
| Bovine | Yes | Yes | Yes* | 6 | |
| Caco-2 (HTB-37) | Human | ? | ? | Yes | 17 |
| Human, bovine | Yes | NR | NR | 24 | |
| Primary rat hepatocyte | Human | Yes | Yes | Yes | 17 |
| L929 (CCL-1) | Human, bovine | Yes | Yes | NR | 34 |
| LGA | Human, bovine | Yes | NR | NR | 10 |
| HT29.74 (HTB-38) | Human | Yes | ? | NR | 22 |
| Intestine 407 (CCL-6) | Bovine | Yes | NR | NR | 30 |
| RL95-2 (CRL-1671) | Bovine | Yes | Yes | ? | 43 |
| T84 (CCL-248) | Bovine | Yes | Yes | NR | 1 |
| BFTE | Bovine | Yes | Yes | Yes | 64 |
| MDBK (CCL-22) | Bovine | Yes | Yes | Yes | 25, 57 |
| HCT-8 (CCL-244) | Bovine | Yes | Yes | Yes | 38, 52-55 |
a
See Table 1 for descriptions of cell lines and abbreviations. See text for culture details.
b
NR, not reported; ND, not detected; ?, not clear from description; Yes*, immature and possible mature forms.