Table 1. Growth of M. barkeri strains in various media.
| Doubling time, h
|
|||
|---|---|---|---|
| Substrate | Wild type | Δmtr::pac-ori-aph | Δmtr::pac-ori-aph hpt::mtrECDBAFGH |
| H2/CO2 | 7.2 ± 0.7 | NG | 9.4 ± 0.3 |
| Ac | 60.1 ± 7.9 | NG | 67.1 ± 9.8 |
| Me | 5.7 ± 0.5 | NG | 10.7 ± 0.5 |
| Me + H2/CO2 | 5.8 ± 1.9 | 5.4 ± 0.5 | 6.0 ± 0.5 |
| Me + Ac | 6.7 ± 0.6 | 13.2 ± 1.2 | 11.9 ± 0.1 |
Growth rate was measured by measuring optical density during growth in HS broth with the indicated substrates; Me, methanol; Ac, acetate. Doubling time (in hours) from at least three independent measurements is reported. Positive cultures, except acetate, typically grew within 3 days; Ac cultures grew within 4 weeks. NG, no growth after incubation for at least 6 months.