Table 2. Methane production by cell suspensions of M. barkeri strains.
M. barkeri
|
M. barkeri Δmtr
|
|||
---|---|---|---|---|
Substrate | CH4 produced, μmol | Rate of CH4 production, nmol·min−1·mg−1 | CH4 produced, μmol | Rate of CH4 production, nmol·min−1·mg−1 |
N2/CO2 | <1 | <1 | <1 | <1 |
H2/CO2 | 308 ± 55 | 221 ± 37 | <1 | <1 |
Me | 350 ± 18 | 328 ± 26 | 109 ± 5 | 9 ± 4 |
Me + H2/CO2 | 691 ± 39 | 731 ± 36 | 442 ± 46 | 422 ± 41 |
Me + Ac | 356 ± 6 | 309 ± 6 | 491 ± 13 | 171 ± 20 |
Assays contained 500 μmol methanol and/or 500 μmol acetate and were conducted as described in Materials and Methods; gas phase was either N2/CO2 (80%/20%) or H2/CO2 (80%/20%) at 250 kPa.