Fig. 10.

Effects of methionine and adenosine on the mobility of human MTHFR in transfected HEK293 cells. (A) Wild-type human MTHFR was expressed in HEK293 cells cultured in modified DMEM containing or lacking methionine or leucine and containing or lacking FBS. Cells were harvested after 12 h of nutrient limitation. (B) Wild-type human MTHFR was expressed in HEK293 cells cultured in modified DMEM containing or lacking methionine, and the effect of additions of adenosine (1 mM) or uridine (1 mM) was examined. Cells were harvested 12 h after the initiation of methionine limitation and/or addition of adenosine or uridine.