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Alkaline phosphatase treatment leads to increased mobility of human MTHFR. (A) SDS/PAGE analysis of purified full-length human MTHFR from Sf9 cells. (B) Analysis of human MTHFR after tryptic cleavage of the native enzyme (0), enzyme as isolated, or after incubation of enzyme with (+) or without (-) addition of alkaline phosphatase for 1 h at 37°C. Molecular mass markers are shown in the rightmost lane of each panel.
