Fig. 4.

Deletion of Xrn1p increases the stability of recombinant RNAs and DI-72 replicon RNA. Four representative recombinant RNAs containing partially duplicated sequences (first four bars on the left) and the DI-72 replicon RNA (the dark gray bar on the right) were separately expressed in the parental (A) and xrn1Δ (B) strains from GAL1 promoter. After repression of transcription with glucose (time points of 0, 2, 4, and 6 h), the residual viral RNAs were measured by Northern blotting and quantified by using a PhosphorImager. The data are shown in % (the amount of viral RNA at 0 time point is 100%) derived from four independent experiments.