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Undefined medium SCGYEM for cultivation of N. fowleria
| Component | Amt or concn |
|---|---|
| Organic | |
| Isoelectric casein | 10.0 g |
| Glucose | 2.5 g |
| Yeast extract | 5.0 g |
| Fetal calf serum | 100.0 ml |
| Penicillin-streptomycin | 200.0 μg/ml (each) |
| Distilled water | 900.0 ml |
| Buffering | |
| Na2HPO4 | 1.325 g |
| KH2PO4 | 0.8 g |
