TABLE 3.

Defined medium DGM-21A for Acanthamoeba spp.^a

Component	Concn
Amino acids (g/liter)
l-Alanine	0.2
l-Asparagine	0.5
l-Aspartic acid	0.3
l-Arginine.HCl	1.0
l-Cysteine	0.2
l-Cystine	0.1
l-Glutamic acid	0.5
l-Glutamine	0.5
Glycine	1.5
l-Histidine.HCl	0.2
l-Isoleucine	0.6
l-Leucine	0.9
l-Lysine · HCl	1.0
l-Methionine	0.3
dl-Phenylalanine	0.9
l-Proline	0.8
l-Serine	0.2
l-Threonine	0.5
l-Tryptophan	0.2
l-Tyrosine	0.2
l-Valine	0.7
Vitamins (mg/liter)
Biotin	0.3
Folic acid	0.2
Ascorbic acid	2.0
Riboflavin	1.0
Thiamine · HCl	0.01
Thioctic (lipoic) acid	0.4
B12	0.01
Salts (g/liter)
CaCl2 · 2H2O	0.0074
MgSO4 · 7H2O	0.25
KH2PO4	0.27
NaHCO3b	0.25
NH4Cl	0.0005
NH4 formate	0.0006
FeSO4 · 7H2O	0.009
Trace elements (mg/liter)
ZnSO4 · 7H2O	1.00
MnCl2 · 4H2O	2.30
(NH4)Mo7O24.4H2O	0.40
CoCl2	0.017
CuSO4	0.0033
H3BO3	0.10
Na2EDTA	0.01
Other components (g/liter)
Glucose	15.0
Na acetate	2.5
Ethanolb	0.5

^aMedium DGM-21A was described previously by Byers et al. (9). Individual groupings of components were prepared as stock solutions. pH before autoclaving, 6.5.

^bIntroduced from vitamin stock solution.