TABLE 4.
Defined medium M-11 for A. culbertsonia
| Component | Concn |
|---|---|
| Amino acids (mg/liter) | |
| l-Arginine · HCl | 825.0 |
| Glycine | 1,500.0 |
| l-Histidine · HCl | 160.0 |
| l-Isoleucine | 600.0 |
| l-Leucine | 900.0 |
| l-Lysine · HCl | 1,250.0 |
| l-Methionine | 300.0 |
| l-Phenylalanine | 900.0 |
| l-Threonine | 500.0 |
| l-Tryptophan | 200.0 |
| l-Valine | 700.0 |
| Other (g/liter) | |
| Glucose | 1.8 |
| Na citrate | 1.0 |
| Vitamins (mg/liter) | |
| Biotin | 0.25 |
| B12 | 0.00125 |
| Thiamine · HCl | 1.25 |
| Salts (g/liter) | |
| CaCl2 · 2H2O | 0.0588 |
| MgSO4 · 7H2O | 0.985 |
| KH2PO4 | 0.340 |
| Na2HPO4 · 2H2O | 0.445 |
| (NH4)2SO4 · FeSO4.6H2O | 0.0196 |
| Trace elements (mg/liter) | |
| MnCl2 · 4H2O | 2.3 |
| ZnSO4 · 7H2O | 1.0 |
| (NH4) Mo7O24.4H2O | 0.4 |
| CuSO4 · 5H2O | 0.0033 |
| CoCl2 | 0.017 |
| H3BO3 | 0.1 |
| Na2EDTA | 0.01 |
a
Medium M-11 was described previously by Shukla et al. (69). Initial pH, 6.5.