TABLE 5.

Complex growth medium BM-3 for B. mandrillaris^a

Component	Amt
Basal medium
Biosate peptone	2.0 g
Yeast extract	2.0 g
Torula yeast RNA	0.5 g
Double-distilled water	345.0 ml
Supplements
Hanks' balanced salts, 10×	34.0 ml
5% Liver digest in Hanks' salts	100.0 ml
MEM vitamin mixture, 100×	5.0 ml
Lipid mixture, 1,000× (Gibco)	0.5 ml
MEM nonessential amino acids, 100×	5.0 ml
10% Glucose	5.0 ml
Hemin, 2 mg/ml	0.5 ml
0.5% Taurine	5.0 ml
Calf serum	50.0 ml

^aMedium BM-3 was described previously in reference 66. pH adjusted to 7.2.