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Recommended subculturing schedules for stock cultures of free-living amebas
| Ameba culture | Type of culture | Culture schedule (wk) at:
|
|||
|---|---|---|---|---|---|
| ∼20°C | Room temperature (∼25°C) | 30°C | 37°C | ||
| Acanthamoeba spp. | Bacterized | 4 | 3-4 | 1-2 | 1 |
| Axenic | 4 | 3-4 | 2-3 | 2 | |
| Naegleria spp. | Bacterized | 4 | 3-4 | 2 | 1-2 |
| Axenic | 2 | 1-2 | 1-2 | 1 | |
| B. mandrillaris | Axenic | 3 | 2-3 | 2-3 | 2 |
| With tissue culture cells | 1 | 1 | |||
