Fig. 4.

Immunofluorescence staining of TLR2 and NOD1 receptors protein in bovine endometrial explants exposed to sperm and semen extender (SE). (A) TLR2 protein expression (red) in bovine endometrial explants exposed to sperm and 10% SE. DAPI was used as the nuclear counterstain (blue), with a merged image showing DAPI in blue and TLR2 in red. Normal rabbit IgG was used as the primary antibody in the IgG control. (B) NOD1 protein expression (green) in bovine endometrial explants exposed to sperm and 10% SE. DAPI was used as the nuclear counterstain (blue), with a merged image showing DAPI in blue and NOD1 in green. Normal mouse IgG was used as the primary antibody in the IgG control. Semiquantitative scoring of corrected total cell fluorescence (CTCF) of TNF and NLRP3 expressed as arbitrary fluorescence units. CTCF values are presented as the mean relative expression ± SEM from three independent experiments. Data were analyzed using the ANOVA with Bonferroni’s mean comparisons procedure. Asterisks indicate significant differences (** P < 0.01). Scale bar = 50 μm.