TABLE 1.
Composition of RPMI 1640 tissue culture mediuma
| Component | Amt (mg/liter) |
|---|---|
| Inorganic salts | |
| Ca(NO3)2 · 4H2O | 100.0 |
| KCl | 400.0 |
| MgSO4 (anhydrous) | 48.8 |
| NaCl | 5,300.0 |
| NaHCO3 | 2,000.0 |
| Na2HPO4 (anhydrous) | 800.0 |
| Amino acids | |
| l-Arginine | 200.0 |
| l-Asparagine (free base) | 50.0 |
| l-Aspartic acid | 20.0 |
| l-Cystine · 2HCl | 65.0 |
| l-Glutamic acid | 20.0 |
| l-Glutamine | 300.0 |
| Glycine | 10.0 |
| l-Hisitidine (free base) | 15.0 |
| l-Hydroxyproline | 20.0 |
| l-Isoleucine | 50.0 |
| l-Leucine | 50.0 |
| l-Lysine · HCl | 40.0 |
| l-Methionine | 15.0 |
| l-Phenylalanine | 15.0 |
| l-Proline | 20.0 |
| l-Serine | 30.0 |
| l-Threonine | 20.0 |
| l-Tryptophan | 5.0 |
| l-Tyrosine · 2Na · 2H2O | 29.0 |
| l-Valine | 20.0 |
| Vitamins | |
| d-Biotin | 0.20 |
| d-Ca pantothenate | 0.25 |
| Choline chloride | 3.0 |
| Folic acid | 1.0 |
| I-Inositol | 35.0 |
| Niacinamide | 1.0 |
| para-Aminobenzoic acid | 1.0 |
| Pyridoxine HCl | 1.0 |
| Riboflavin | 0.20 |
| Thiamine HCl | 1.0 |
| B12 | 0.005 |
| Other components | |
| d-Glucose | 2,000.0 |
| Glutathione (reduced) | 1.0 |
| HEPES | 5,958.0 |
| Phenol red | 5.0 |
a
From the GIBCO BRL product catalog. Formula for 25 mM HEPES-buffered liquid RPMI 1640 at 1× concentration, with l-glutamine. Components and concentrations may vary with the state of the medium: 1× or 10×, liquid or powder, with or without glutamine, or with or without HEPES buffering.