TABLE 2.

In vitro cultivation of stages of human Plasmodium spp.^a

Stage	Species	Basal medium(a)	Additional factors	Reference(s)
Exoerythrocytic	P. falciparum	MEM	Hepatoma cells	11
		RPMI 1640-MEM		43
	P. malariae	MEM	Chimpanzee primary hepatocytes	56
	P. ovale	Arginine-free Ham's medium	Human and rat hepatocytes	53
	P. vivax	MEM	Hepatoma cells	28
Erythrocytic	P. falciparum	RPMI 1640		84, 85
		Semidefined medium		17
		RPMI 1640	Cell-free system with Matrigel	89, 98
		Malaria culture medium	Feeder cells (C. fasciculata)	3
	P. malariae	RPMI 1640	Glutamine, hypoxanthine	48
	P. vivax	SCMI 612	Elevated glucose	9
		RPMI 1640	Reticulocytes	58, 59
		McCoy's 5A	Reticulocytes	22
Gametocyte	P. falciparum	RPMI 1640	Hypoxanthine	33
		RPMI 1640	Hormonal stimulation (insulin, etc.)	49, 50
Sporogonic	P. falciparum	RPMI 1640	Drosophila cells and Matrigel	94

^aSummary of the different media used for cultivation of the several species of the malaria parasite and their different stages.