TABLE 3.
In vitro cultivation of stages of non-human Plasmodium spp.a
| Stage | Species | Basal medium(a) | Additional factor | Reference(s) |
|---|---|---|---|---|
| Exoerythrocytic | P. berghei | L-15 | Rodent liver cells | 19 |
| MEM; NCTC-135 | Human embryonic lung or hepatoma cells | 29, 30 | ||
| P. cynomolgi | MEM | Primary hepatocytes | 57 | |
| P. fallax | Medium 199-LDGM | Turkey embryos | 16 | |
| P. yoellii | MEM | Human hepatoma cells | 12 | |
| Erythrocytic | P. berghei | RPMI 1640 | 60 | |
| P. chabaudi | BME; William's medium | 63 | ||
| P. cynomolgi | Rhesus erythrocytes | 62 | ||
| P. lophurae | Duck erythrocyte extract | 46 | ||
| Sporogonic | P. berghei (oocysts) | RPMI 1640 | With or without insect cells, fetal bovine, and silkworm sera | 76 |
| P. berghei (ookinetes) | MEM | 95 | ||
| P. gallinaceum | RPMI 1640 | Fetal bovine serum plus Drosophila cells and Matrigel | 93 | |
| P. relictum (sporozoites and ookinetes) | Chicken serum and embryo extract | 4, 5 |
a
Summary of the different media used for cultivation of the several species of the malaria parasite and their different stages.