TABLE 2.

Medium for cultivation of feeder layer fibroblast-like cells and bloodstream-stage trypanosomes^a

Component	Amt
MEM with Earle's salts without l-glutamine	450.0 ml
Double-distilled water	45.0 ml
MEM nonessential amino acids, 100×	5.0 ml
HEPES	3.6 g
Glucose	1.0 g
l-Glutamine	150.0 mg
Heat-inactivated rabbit serum	15.0%

^aMedium described previously by Brun et al. (17). pH 7.5 after adjustment with 4 N NaOH