TABLE 4.
Serum replacement supplement for cultivation of T. brucei and T. evansia
| Component | Concn (mg/liter) |
|---|---|
| d-Biotin | 1.0 |
| Retinol | 0.1 |
| β-Alanine | 80.0 |
| l-Anserine nitrate salt | 17.0 |
| o-Phosphorylethanolamine | 30.0 |
| Sarcosine | 57.0 |
| l-Ornithine hydrochloride | 3.0 |
| Taurine | 6.0 |
| ATP · Na2 | 5.5 |
| 2′-dCMP | 3.5 |
| 2′-dGMP | 3.5 |
| 5-Methyltetrahydrofolic acid | 0.5 |
| Bovine α2-macroglobulin | 0.2 |
| Bovine β-lipoprotein | 0.1 |
| Fatty acid-free bovine serum albumin | 5,000.0 |
a
Serum replacement supplement was described previously by Hirumi et al. (48).