TABLE 5.
Enriched medium F-69 for growth of T. cruzi amastigotes and epimastigotesa
| Component | Amt |
|---|---|
| Basal medium | |
| Distilled water | 22.0 ml |
| Medium 199, 10× | 10.0 ml |
| 0.8% glucose | 25.0 ml |
| 0.1 M sodium pyruvate | 2.0 ml |
| 5% Trypticase | 10.0 ml |
| Vitamin mixtureb | 1.0 ml |
| Biotin (2 mg/ml) | 1.0 ml |
| Folic acid (10 mg/ml) | 5.0 ml |
| Vitamin B12 (1 mg/ml) | 1.0 ml |
| 4% methyl cellulose | 5.0 ml |
| Hemin (50 mg/ml; in 0.01 N NaOH) | 5.0 ml |
| Penicillin G-streptomycin (100,000 U/ml) | 0.1 ml |
| ATP, ADP, AMP solutionb | 10.0 ml |
| 4.2% NaHCO3 | 3.0 ml |
| Vitamin mixture | |
| p-Aminobenzoic acid | 30 mg |
| d-Calcium pantothenate | 40 mg |
| Choline chloride | 30 mg |
| Isoinositol | 30 mg |
| Nicotinamide | 50 mg |
| Nicotinic acid | 20 mg |
| Pyridoxal · HCl | 20 mg |
| Pyridoxine · HCl | 20 mg |
| Pyridoxamine · 2HCl | 20 mg |
| Riboflavin-5-phosphate-Na · 2H2O | 4 mg |
| Thiamine | 20 mg |
| Distilled water | 100 ml |
| ATP, ADP, AMP solution | |
| ATP | 50 mg |
| ADP | 10 mg |
| AMP | 10 mg |
| Glutathione (reduced) | 20 mg |
| l-Cysteine · HCl | 5 mg |
| Ascorbic acid | 5 mg |
| Distilled water | 100 ml |
a
Enriched medium F-69 was described previously by S. Pan (77).
b
Described in this table.