TABLE 6.

Defined medium HOSMEM-II for cultivation of L. donovani promastigotes^a

Componentb	Amt
MEM from powder	10.58 g
NaHCO3	1.0 g
MEM amino acids, 50×	10.0 ml
MEM nonessential amino acids, 100×	10.0 ml
1% Na pyruvate	11.0 ml
30 mM MOPSd	6.28 g
Glucose	2.0 g
Biotin	0.1 mg
p-Aminobenzoic acid	1.0 mg
Solution Ac	10.0 ml
Solution Bc	2.5 ml
Double-distilled water	800.0 ml
Solution A
Hypoxanthine	150 mg
Ascorbic acid	2 mg
Vitamin B12	2 mg
Fatty acid-free bovine albumin fraction IV	150 mg
Thioctic acid	4 mg
Menadione	4 mg
Retinol acetate	4 mg
Double-distilled water	91 ml
Solution B
Hemin	250 mg
Folic acid	500 mg
0.05 N NaOH	50 ml
Double-distilled water	50 ml

^aMedium HOSMEM-II was described previously by Berens and Marr (14).

^bFor components apart from those in solutions A and B, pH was adjusted to 7.2 with 5 N NaOH, volume was brought to 1 liter, and the mixture was filter sterilized. Solution B was added prior to use of the medium. The pH of the medium was adjusted to 8 with 1 N HCl.

^cDefined in this table.

^dMOPS, morpholinepropanesulfonic acid.