Figure 7.

Substance P signalling is required for ASP7663-induced mechanical hypersensitivity. (A) Example recording showing afferent firing during 3 consecutive ramp distensions of the colon, with SP, thiorphan, and captopril applied between the second and third ramps. (B) Grouped data showing the percentage change in afferent firing during the third ramp distension after pretreatment with either thiorphan and captopril alone or thiorphan and captopril with 15 µM of SP. Two-way repeated measures ANOVA with Holm–Sidak post-hoc tests. (C) Grouped data showing the peak percentage change in afferent firing from the experiments shown in (A and B). Two-tailed unpaired t test. (D) Example recording showing afferent firing during 3 consecutive ramp distensions of the colon with aprepitant and ASP7663 applied between the second and third ramps. (E) Grouped data showing the percentage change in afferent firing during the third ramp distension after pretreatment with ASP7663 with or without aprepitant. Two-way repeated measures ANOVA with Holm–Sidak post-hoc tests. (F) Grouped data showing the peak percentage change in afferent firing from the experiments shown in (D and E). Two-tailed unpaired t test. (G) Example recording showing afferent firing during 3 ramp distensions with aprepitant alone applied between the second and third ramps. (H) Grouped data showing the change in afferent firing rate during the second (preaprepitant) and third (postaprepitant) ramp distensions. Two-way repeated measures ANOVA with Holm–Sidak post-hoc tests. (I) Grouped data showing the peak change in afferent firing rate during the second (preaprepitant) and third (postaprepitant) ramp distensions. Two-tailed paired t test. SP, substance P.