Table 3.

Comparison of the detection limits of the two molecular assays

DNA concentration	PCR	AcanITS1 qPCR
100 ng/μl	+	+
10 ng/μl	+	+
1 ng/μl	−	+
100 pg/μl	−	+
10 pg/μl	−	+
1 pg/μl	−	−

+, positive; −, negative