TABLE 1.
Cell cycle parameters were obtained by analyzing the rate of BrdU incorporation. The total cell cycle length (TC), S-phase duration (TS), and the duration of cell cycle phases G0/G1 and G2/M (TC–TS) are provided in hours. The ratio between the S-phase and total cell cycle length (TS/TC) and the growth fraction (GF) are expressed as relative values. One-way ANOVA with Bonferroni adjusted post-hoc t-tests. Independent experiments: n = 7 (physioxia), n = 7 (continuous hyperoxia), n = 6 (short-term hyperoxia).
| TC (h) | TS (h) | TC - TS (h) | TS/TC | GF | |
|---|---|---|---|---|---|
| Physioxia | 15.81 ± 0.95 | 5.01 ± 0.40 | 10.8 ± 0.56 | 0.31 ± 0.02 | 0.94 ± 0.50 |
| Continuous Hyperoxia | 26.00 ± 3.07 | 8.22 ± 1.46 | 17.79 ± 1.61 | 0.30 ± 0.01 | 0.92 ± 0.52 |
| Short-Term Hyperoxia | 7.08 ± 0.37 | 2.80 ± 0.25 | 4.27 ± 0.12 | 0.39 ± 0.01 | 0.94 ± 0.54 |
| One-way ANOVA | p < 0.001, F-value = 22.7 | p = 0.003, F-value = 8.4 | p < 0.001, F-value = 40.9 | p = 0.002, F-value = 8.9 | p = 0.051, F-value = 3.6 |
| Bonferroni adjusted post-hoc Student’s t-test | |||||
| Physioxia vs Continuous Hyperoxia | p = 0.005 | p = 0.070 | p = 0.012 | p = 1.000 | p = 0.094 |
| Physioxia vs Short-Term Hyperoxia | p = 0.02 | p = 0.350 | p < 0.001 | p = 0.011 | p = 1.000 |
| Continuous Hyperoxia vs Short-Term Hyperoxia | p < 0.001 | p = 0.003 | p < 0.001 | p = 0.003 | p = 0.082 |