Fig. 2.

ORMOSIL nanoparticle transfection in the SNc. (A) DNA-free ORMOSIL injection showing no substantial immunostaining for EGFP. (B–E) Injection of ORMOSIL-pEGFP-N2 complex into SNc. (B) Multiple cells with typical dopaminergic neuron morphology are immunostained positive for EGFP. (C) No immunostaining is observed without primary anti-EGFP Ab. (D) EGFP immunostaining of neuron-shaped cells (higher magnification). (E) Transfected EGFP (green) is expressed in TH-immunopositive (red) dopaminergic neuron.