FIG. 7.

K-bZIP association with Ubc9. K-bZIP expressed in BCBL-1 was coprecipitated with Ubc9 by Ubc9 antibodies. Anti-HA antibody was used as a negative control. (B) Association between K-bZIP and Ubc9 in cotransfected 293T cells. 293T cells were cotransfected with the indicated plasmids. Cell lysates were precipitated with Flag antibody-conjugated agarose, and coimmunoprecipitation of K-bZIP was detected by using anti-T7 tag antibody. The expression of T7-tagged K-bZIP in one-tenth of total cell lysates used in coimmunoprecipitation is shown in the same blot as a control. (C) K-bZIP associated with Ubc9 in vitro. GST-Ubc9 but not GST precipitates in vitro-translated (IVT)-K-bZIP. The inputs are one-tenth of the lysates used for the binding study.