TABLE 2.

Primers used for mutagenesis

Primer	Sequence (5′-3′)a
SUMO-1GG-F	ACG GGA GGT TAA TCA ACA GTT TAC GGT CCG TGA
SUMO-1GG-R	AAC TGT TGA TTA ACC TCC CGT TTG TTC CTG ATA
SUMO-2GG-F	CG GGA GGT TAA TAC TGA CGG TCC GTG AAT TCA TC
SUMO-2GG-R	CCG TCA GTA TTA ACC TCC CGT CTG CTG TTG GAA C
SUMO-3GG-F	ACG GGA GGT TAG CCG GAG AGC AGC CTG GCA GG
SUMO-3GG-R	GCT CTC CGG CTA ACC TCC CGT CTG CTG CTG GAA C
SENP1 RK/LM	CCA TAC TTC CTGATG CGG ATG GTC TGG GAG
SENP1 RK/LM	CTC CCA GAC CAT CCG CATCAG GAA GTA TGG
K-bZIP158K/R-F	TCT GTA GTT AGG GCC GAA GTA TGT GAT CAG TCA
K-bZIP 158K/R-R	TTC GGC CCT AAC TAC AGA CGC AGG CAC G
Ubc9 C93S-F	GGG ACA GTG TCC CTG TCC ATC TTA GAG GAG GA
Ubc9 C93S-R	GGA CAG GGA CAC TGT CCC CGA AGG GTA CAC AT

^aA mutation was introduced in the underlined sequence.