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Effect of added divalent cations on PBCV-1 dUTPase activity
| Added cation (concn [mM]) | Relative activitya (%) with:
|
|
|---|---|---|
| No EDTA | 1 mM EDTA | |
| None | 80 | 0 |
| Mg2+ (5) | 100 | 100 |
| Mn2+ (5) | 71 | 70 |
| Co2+ (5) | 61 | 59 |
| Zn2+ (5) | 52 | 49 |
| Ni2+ (5) | 47 | 30 |
| Ca2+ (5) | 38 | 31 |
| Cu2+ (5) | 32 | 28 |
The results are expressed as the percentage of enzyme activity relative to the activity obtained with Mg2+. The results are the average of three independent experiments.
