FIG. 1.
Unprocessed images (magnification, ×184) from fluorescent microscopy of various cell lines after 8 h of infection with HSV1716gfp propagated either in BHK (a to h, o to r) or Vero (i to n) cells. Permissive BHK (a, i), C8161 (b, j), HeLa (c, k), or SK-N-SH (d, l) cells were infected with 5 × 106 PFU HSV1716gfp. Nonpermissive CHO cells were infected with 1 × 106 PFU, 5 × 106 PFU, or 1 × 107 PFU HSV1716gfp/BHK (e, f, g, respectively) or with 1 × 107 PFU HSV1716gfp/Vero (m). Nonpermissive mouse melanoma cells were infected with 1 × 107 PFU HSV1716gfp/BHK (h) or HSV1716gfp/Vero (n). Infection of BHK (o, p) or CHO (q, r) cells was completely neutralized using a sheep anti-HSV-1 antiserum (o and q, respectively) but was unaffected by normal sheep serum (p and r, respectively).